Rapid species-identification and antibiotio susceptibility test with normal flora isolated from patients with recurrent bronchitis or tracheobronchitis are important not only for the ideal chemotherapy but also for the prevention from pneumonia.
In
this
sutudy, the species of normal flora isolated from sputa of 22 patiens with chronic bronchitis were identified by cultural and biochemical properties and antibiotic sensitivities were investigated by disk diffusion method. In addition the
significance of
the direct Gram smear examination with sputum was discussed.
The most frequent species of normal flora. Isolated from sputa of patients with chronic bronchitis were K. pneumoniae(59.0%), followed by P. aeruginosa(50%), S. pneumoniae(40.9%), H. influenzae and Neisseria spp. (each 27.2%), S. aureus, and C.
albicans(each 13.6%), B.catarrhalis and P. multocida(each 9.0%), and P. gallinarum(4.5%). Respectively. The direct Gram's stain of sputum was useful in the diagnosis of infections with S. pneumoniae, H. influenzae, S. aureus, and C. albicans, but
the
cultural method was required for diagnosis of infections with other species.
All isolated of S. pneumoniae were susceptible to vancomycin, K. pneumoniae to norfloxacin, P. aeruginosa to norfloxacin, axtreonam and cefoperazone. Pasteurella spp. to cephalothin and tetracycline, B. catarrhalis and N. flavescens to
erythromycin
and
tetracycleine, respectively, and C. albicans to sporanox.
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